Imaging Facility 2020-11-11T10:09:18-05:00

Oscar Imaging Facility

COVID-19 UPDATE:

You will find the most current up to-date information of the OSCAR imaging facilities response to COVID-19 on our web page.

Current Research phase: PHASE 3

We understand during these trying times many researchers, and students are eager to continue their research. Therefore, the OSCAR Imaging Facility is open during the COVID19 Pandemic. We are following University, State and local guidelines in order to keep the facility operational. However, the facility is only open for graduate students, postdocs, and lab technicians. Absolutely no undergraduates are permitted to enter or use the equipment at this time.

If you wish to use the facilities, this includes the Imaging suite, wetlab, spectroscopy and Image Analysis rooms, please contact Mr. Michael Moore, mmoore@desu.edu prior to booking your time on the iLab calendar.

UPDATE: 11/11/2020 KN95 Masks are now available for use in the Imaging Facility and Wet-Lab. KN95 masks are only to be used for one-on-one appointments. Please contact Mr. Moore for more details.

General guidelines for restarting research this summer and as we move into the fall. 

Phase 3:
Campus and room occupancy limited as in phase 2, social distancing requirements remain as phase 1.

  1. Training and research requiring close proximity – Instrument training, joint data collection and
    other activities that require close physical proximity between researchers may resume under the
    following conditions. Individuals who will be working in close contact must wear N95, KN95 or
    similar masks, and lab coats for the duration of the time in which they are in close contact.
    Acceptable face coverings include N95or KN95 masks or a combination of a surgical mask with
    face shield. PIs are responsible for ensuring that people who will be working in close contact
    have access to N95 masks and lab coats.

About Us:

The OSCAR Imaging Facility at Delaware State University (DSU) is a multi-user core facility that offers state-of-the-art equipment for imaging, microscopy, spectroscopy, and image analysis. The imaging facilty is open to all university researchers and industrial collaborators on a fee-based service. The Imaging Suite is located in room A124 of the Imaging Suite of the OSCAR building.

The OSCAR Imaging facility is an outgrowth of the NSF-CREST program started at DSU in 2005, and supported by the current CREOSA/OSCAR Center at DSU. It has evolved into a major resource for DSU investigators and, more importantly, for training students undergraduate and graduate in imaging techniques, and motivating inter-disciplinary research among its users.

To create an account on our Calendar: ILAB https://my.ilabsolutions.com/account/signup/539?sc_id=4169

-To login to your ILAB account: https://my.ilabsolutions.com/account/login

Equipment: OSCAR Building, Imaging Facility Suite, Room A124

Scanning Electron Microscope

SEM micrograph of Diatom, Blackbird Creek Delaware, Ozbey Lab

FEI Quanta 250 FEG

Field Emission Gun Scanning Electron Microscope for Characterization of conductive and non-conductive samples. high vacuum, low vacuum, and Environmental SEM capable. in situ anyalysis up to 1000oC with heating stage

Features:

  • Everhardt Thornley SED (secondary
  •  Large Field Low vacuum SED (LFD)
  • Gaseous SED (GSED) (used in ESEM)
  • Concentric Backscattered Detector (CBS)
  • Oxford 80mm EDS Detector with Aztec software (offline analysis)
  • Oxford Nordlys EBDS
  • Motorized Stage with Tilt and rotation

Zeiss LSM510 Confocal

Zeiss LSM510 Confocal Inverted Microscope Equipped for multi-color acquisition (up to 3 Channels plus Transmitted) 458, 488, 543, 633 Laser Lines.

Features:

  • DIC
  • ZEN 2009 Software
  • Motorized Stage
  • Live Cell Incubation
  • FRET / FRAP
  • Can not image DAPI

Objectives:

  • 10x 0.45NA
  • 20x 0.75NA
  • 40X 1.3NA oil
  • 63x 1.4NA oil
  • 40x 1.2NA water
  • 63x 1.2Na water


Leica TCS-SP5 Confocal

Leica TCS-SP5 Confocal. Equipped for multi-color acquisition (up to 3 channels plus transmitted). 405, 458, 488, 514, 543 and 633 laser lines

Features:

  • Bright field
  • Manual Stage
  • Z-stack
  • Time-lapse
  • FRET/FRAP
  • Spectral Detection

Objectives:

  • 10x 0.35NA
  • 20x 0.75NA
  • 40X 0.75NA
  • 40x 1.3NA oil
  • 63x 1.4NA oil
  • 63x 1.2Na water

Fluorescence and DIC Microscope

Olympus IX71 widefield inverted microscope. High sensitivity camera (Photometrics QuantEM 512C), Computer controlled motorized stage.

  • Multi-channel epifluorescence
  • Phase Contrast
  • Bright field
  • Z-stack
  • Time-lapse
  • DIC

Location: OSCAR Building, Imaging Facility Suite, Room A124


Widefield Polarization Microscope

Polarized light microscopy Fly wing

Olympus BX53, upright polarizing microscope. Q camera software & MicroManger

Features:

  • Polarization
  • De Sénarmont compensator
  • 360o Stage with 1o increments
  • Q-Imaing QuickCAM cooled 12Bit Color CCD Camera
  • Time laps

Objectives

  • 5x 0.13NA Pol
  • 10x 0.30NA Pol
  • 20x 0.50NA Pol
  • 40X 0.75NA Pol
  • 100X 1.25 NA oil

Atomic Force Microscope

AFM: Silica Deposition Thin film

Bruker Innova SPM AFM system, with 5 um to 90 um area scanner.

  • Contact mode
  • Tapping Mode
  • STM
  • Lateral Force Microscopy
  • Phase Imaging
  • Liftmode
  • Magnetic Force
  • Electric Field Microscopy
  • Surface Potential
  • Liquid Imaging in contact and tapping mode

Location: OSCAR Building, Imaging Facility Suite, Room A124

Cross Correlation Spectro-Fluorometer

ISS K2 phase + modulation spectro-fluorometer.

  • Steady state and time-resolve fluorescent emission and absorption measurements


Transmission Spectro-Photometer

Varian Cary 100 BIO spectrometer for UV-visible light.

  • Absorbance and transmittance, referenced measurements, multiple scan option

Image Analysis Room

Computers:

  • Dell Precision T7610
    • Windows 10
    • Eight 3.4GHz cores
    • 12 GB NVIDIA Quadro K6000
    • 128 GB RAM
  • Dell Precision T5810
    • Windows 10
    • 4 GB NVIDIA GeForce GTX 980
    • 64 GB Ram
  • Four Dell T3610 Work Stations
  • Windows 10
  • ImageJ, Word, Excel
  • For student use

Software

  • Imaris 9.5
  • Avizo Wind 8.1
  • Huygens Deconvolution
  • ZEN Black
  • ICY
  • ImagJ FIJI

Wet Lab

Capabilities/Equipment

  • Cell Culture
  • Electron and Light Microscopy sample prep equipment
  • Cell Counter
  • Plasma Etcher
  • Gold / Carbon Coater


Policies

For the full version of the center policies (including the fee schedule), please contact us.

1. Hour of operations: 9AM-4PM Monday to Friday

2. Equipment Scheduling:

  • To schedule an appointment on an instrument please use our Calendar:  ILAB
  • To create an account on our Scheduling software: Sign-Up

3. Acknowledgments / Co-authorship Policy:

3.1 Acknowledgments
Investigators are requested to recognize the role of the OSCAR Imaging facility in producing data for publication in the acknowledgement section of manuscripts and posters presented to meetings and conferences. This is critical of demonstrating the importance of the imagingfacility to the research conducted at Delaware State University and with our external collaborators and will help to secure additional equipment and funding. To keep track of publications linked to the imaging facility, authors should submit a copy of the manuscript and the poster as a pdf to the director of imaging.

3.2 Co-authorship
Co-authorship agreements will be made before the commencement of the study and staff member included as an author should have the opportunity to review the manuscript prior to publication. Co-authorship may be requested in the following instances:
– Significant intellectual contribution to the experiment
– Extensive analysis and/or interpretation of data
– Preparation of data for the purpose of publication.


Facility Director

Hacene Boukari, PhD

Associate Professor
Imaging Facility Director
Department of Physics and Engineering
Delaware State University
Dover, DE 19901
302.857.6511
hboukari@desu.edu

For use of and/or information on the instruments as well as the imaging center, please contact:

Mr. Michael Moore
Imaging Facility Manager
OSCAR Building, Room A104
Delaware State University
Dover, DE 19901
Email: mmoore@desu.edu
Phone: (302) 857-7035